Tryptophan Synthase alpha Chain/Trp A Protein

Catalog No. ABIN7320984

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for Tryptophan Synthase alpha Chain/Trp A Protein (ABIN7320984)

Target: Tryptophan Synthase alpha Chain/Trp A

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant E.coli Tryptophan Synthase α Chain/Trp A Protein

Sequence: Met1-Ser268

Characteristics: Recombinant E.coli Tryptophan synthase alpha chain is produced by our E.coli expression system and the target gene encoding Met1-Ser268 is expressed.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Comment:

29 kDa

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Tryptophan Synthase alpha Chain/Trp A

Background:

Background: Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.

Synonym: Tryptophan synthase alpha chain, trpA,

Molecular Weight: 28.7 kDa

UniProt: P0A877